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Hospital Santo Amaro
Referência em obstetrícia, neonatologia e cirurgias em geral
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IBIT
Matriz da FJS e destaque nacional no combate à tuberculose
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Soluções em Saúde para Empresas
Referência em soluções que garantem a proteção e saúde dos trabalhadores, promovendo um ambiente seguro e sustentável para o futuro da sua empresa.
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Laboratório José Silveira
Qualidade e excelência em análises clínicas e anatomia patológica
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IBR
Modelo em reabilitação de casos de limitações psicomotoras
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Hospital Cristo Redentor
Atende a demanda de partos e de emergências em Itapetinga (BA)
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Centro de Reabilitação da Ribeira
Atendimento especializado a pacientes com deficiências
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Hospital Geral de Itaparica
Atendimento de urgência, obstétrico e cirúrgico
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Santa Casa de Jequié
Qualidade em assistência obstétrica e clínica em Jequié (BA)
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Saúde e Cidadania
Programa que leva saúde e assistência social a quem mais precisa
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NICSA - Memorial em Santo Amaro
Memorial em Santo Amaro
ABSTRACT: BACKGROUND: The initial response to Leishmania parasites is essential in determining disease development or resistance. In vitro, a divergent response to Leishmania, characterized by high or low IFN-γ production has been described as a potential tool to predict both vaccine response and disease susceptibility in vivo.
METHODS AND FINDINGS: We identified uninfected and healthy individuals that were shown to be either high- or low IFN-γ producers (HPs and LPs, respectively) following stimulation of peripheral blood cells with Leishmania braziliensis. Following stimulation, RNA was processed for gene expression analysis using immune gene arrays. Both HPs and LPs were shown to upregulate the expression of CXCL10, IFI27, IL6 and LTA. Genes expressed in HPs only (CCL7, IL8, IFI44L and IL1B) were associated with pathways related to IL17 and TREM 1 signaling. In LPs, uniquely expressed genes (for example IL9, IFI44, IFIT1 and IL2RA) were associated with pathways related to pattern recognition receptors and interferon signaling. We then investigated whether the unique gene expression profiles described here could be recapitulated in vivo, in individuals with active Cutaneous Leishmaniasis or with subclinical infection. Indeed, using a set of six genes (TLR2, JAK2, IFI27, IFIT1, IRF1 and IL6) modulated in HPs and LPs, we could successfully discriminate these two clinical groups. Finally, we demonstrate that these six genes are significantly overexpressed in CL lesions.
CONCLUSION: Upon interrogation of the peripheral response of naive individuals with diverging IFN-γ production to L. braziliensis, we identified differences in the innate response to the parasite that are recapitulated in vivo and that discriminate CL patients from individuals presenting a subclinical infection.
- Data de Publicação: 21/11/2016
- Autores: Carneiro MW, Fukutani KF, Andrade BB, Curvelo RP, Cristal JR, Carvalho AM, Barral A, Van Weyenbergh J, Barral-Netto M, de Oliveira CI. PLoS Negl Trop Dis.