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Hospital Santo Amaro
Referência em obstetrícia, neonatologia e cirurgias em geral
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IBIT
Matriz da FJS e destaque nacional no combate à tuberculose
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Soluções em Saúde para Empresas
Referência em soluções completas e personalizadas em Segurança do Trabalho, Saúde Ocupacional e Gestão Ambiental.
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Laboratório José Silveira
Qualidade e excelência em análises clínicas e anatomia patológica
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IBR
Modelo em reabilitação de casos de limitações psicomotoras
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Hospital Cristo Redentor
Atende a demanda de partos e de emergências em Itapetinga (BA)
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Centro de Reabilitação da Ribeira
Atendimento especializado a pacientes com deficiências
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Hospital Geral de Itaparica
Atendimento de urgência, obstétrico e cirúrgico
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Santa Casa de Jequié
Qualidade em assistência obstétrica e clínica em Jequié (BA)
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Saúde e Cidadania
Programa que leva saúde e assistência social a quem mais precisa
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NICSA - Memorial em Santo Amaro
Memorial em Santo Amaro
ABSTRACT: BACKGROUND:Mycobacterium tuberculosis infection is known to cause inflammation and lung tissue damage in high-risk populations. Nevertheless, direct associations between mycobacterial loads, systemic inflammation and pulmonary lesions upon treatment initiation have not been fully characterized. In the present exploratory study, we prospectively depict the immune profile, microbial clearance and evolution of radiographic lesions in a pulmonary tuberculosis (PTB) patient cohort before and 60 days after anti-tuberculous treatment (ATT) initiation
METHODS: Circulating levels of cytokines (IL-2, IL-4, IL-6, IL-10, IFN-γ, TNF-α) and C-reactive protein (CRP), as well as values of erythrocyte sedimentation rate (ESR) were measured in cryopreserved serum samples obtained from 73 PTB patients at pre-ATT and day 60 of treatment. Changes of the immune profile over time were compared with mycobacterial loads in sputum and culture conversion at day 60 of ATT. Additional analyses tested associations between improvement of chest radiographic lesions at day 60 and pre-treatment status of inflammation and mycobacterial loads.
RESULTS: Within the inflammatory parameters evaluated, values of CRP, IL-2, IL-4, TNF-α and ESR significantly decreased upon treatment initiation. On the converse, IL-10 levels substantially increased at day 60 of ATT, whereas concentrations of IL-6 and IFN-γ remained unchanged. Multidimensional analyses revealed that ESR, IL-2, IL-4 and CRP were the parameters with the highest power to discriminate individuals before and after treatment initiation. We further demonstrated that higher bacterial loads in sputum at pre-ATT were associated with increased systemic inflammation and higher risk for positive M. tuberculosis sputum cultures at day 60 of treatment. Furthermore, we found that pre-ATT mycobacterial loads in sputum and systemic inflammation synergistically associated with the status of radiographic lesions during treatment (Relative risk for chest X-ray improvement: 10.0, 95 % confidence interval: 2.4-40.0, P = 0.002).
CONCLUSIONS: M. tuberculosis loads in sputum are directly associated to the status of systemic inflammation and potentially impact the immune profile, culture conversion and evolution of lung lesions upon ATT initiation.
KEYWORDS: Acid-fast bacilli; Anti-tuberculous treatment; Biomarker; C-reactive protein; Erythrocyte sedimentation rate; Inflammation; Radiographic evaluation; Tuberculosis
- Data de Publicação: 05/08/2016
- Autores: Associations between systemic inflammation, mycobacterial loads in sputum and radiological improvement after treatment initiation in pulmonary TB patients from Brazil: a prospective cohort study. Mesquita ED, Gil-Santana L, Ramalho D, Tonomura E, Silva EC, Oliveira MM, Andrade BB, Kritski A; Rede-TB Study group. BMC Infect Dis.