Abstract

Methods: This implementation study was performed within the RePORT-Brazil consortium between 2016 and 2019 across five research centers. The study described the operational challenges and solutions developed during the transition from QFT-GIT to the QuantiFERON-TB Gold Plus (QFT-Plus) assay. Two collection setups were compared: Setup A (same-site collection and processing) and Setup B (different locations requiring vehicle transport).

Results: Setup B centers experienced a higher percentage of indeterminate results and significantly more nonconformities, such as temperature deviations and longer transport times, compared to Setup A ($P < 0.001$). Temperature deviation was identified as the main nonconformity, particularly during spring and summer quarters. Over time, indeterminate results and nonconformities tended to decrease as the site personnel gained technical expertise.

Conclusion: Successful IGRA implementation requires rigorous standardization of preanalytical steps, regular technical training, and constant monitoring of transport time and temperature. These lessons facilitate large-scale LTBI screening in high TB-burden countries.

Keywords: tuberculosis, IGRA, QuantiFERON-Plus, LTBI, screening, quality control.

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  • Data de Publicação: 01/12/2021
  • Autores: Allyson G. Costa, a,b,c,d Brenda K. S. Carvalho, a,b Mariana Araújo-Pereira, e,f,g Hiochelson N. S. Ibiapina, a,b Renata Spener-Gomes, a,b Alexandra B. Souza, a,b Adriano Gomes-Silva, h Alice M. S. Andrade, e,g Elisangela C. Silva, j,k,l María B. Arriaga, e,f,g Aline Benjamin, h Michael S. Rocha, g,i Adriana S. R. Moreira, j Jamile G. Oliveira, m Marina C. Figueiredo, n Megan M. Turner, n Betina Durovni, h,m Solange Cavalcante, h,m Afranio L. Kritski, j Valeria C. Rolla, h Timothy R. Sterling, n Bruno B. Andrade, e,f,g Marcelo Cordeiro-Santos, a,b,o RePORT-Brazil Consortium

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